Researchers with the U.S. Department of Energy’s Joint BioEnergy Institute (JBEI) have made new strides toward the development of advanced biofuels that can replace gasoline, diesel and jet fuels with a domestically-produced “green” alternative.
Researchers have engineered the first strains of Escherichia coli bacteria, commonly referred to as E. coli, that can digest switchgrass biomass and synthesize its sugars into all three of those transportation fuels. Moreover, the microbes are reportedly able to do this without any help from enzyme additives.
“This work shows that we can reduce one of the most expensive parts of the biofuel production process, the addition of enzymes to depolymerize cellulose and hemicellulose into fermentable sugars,” said Jay Keasling, CEO of JBEI and leader of this research. “This will enable us to reduce fuel production costs by consolidating two steps — depolymerizing cellulose and hemicellulose into sugars, and fermenting the sugars into fuels — into a single step or one pot operation.”
The JBEI researchers attribute the success of this work to the “unparalleled genetic and metabolic tractability” of E. coli. However, the researchers believe that the techniques used in this demonstration should also be adapted to other microbes, which officials say would open the door to the production of advanced biofuels from lignocellulosic feedstocks that are ecologically and economically appropriate to grow and harvest anywhere in the world.
For more information on the researchers’ work with E. coli, click here.
Researchers engineer E. coli to make fuels
Officials at the U.S. Department of Energy’s Joint BioEnergy Institute have engineered the first strains of the bacteria that can digest switchgrass biomass and synthesize its sugars into advanced biofuels to replace gasoline, diesel and jet fuels. The microbes are reportedly able to do this without enzyme additives.
More Alternative Fuels

Alt-Fuel Moves: Product Innovations & Funding Outlooks
Check out some of the latest moves where alternative fuels and school buses intersect, including electric and propane bus deployments, new EV products, and an update from CARB.
Read More →3 New Ways Fleet Software Pays: ROI opportunities for modern fleet managers
Keeping buses safe, reliable, and on schedule requires more than manual processes. This eBook explores how modern fleet software supports school transportation teams with automated maintenance scheduling, smarter video safety tools, and integrated data systems. Discover practical ways fleets are reducing breakdowns, improving safety, and saving valuable staff time.
Read More →
Alt-Fuel Moves: Manufacturing Growth & Energy Storage Expansion
Check out some of the latest moves where alternative fuels and school buses intersect, including manufacturing expansions, major funding awards, and energy storage strategies.
Read More →
Electric School Bus Financing: Making Fleet Transitions Operationally Sustainable for the Long Haul
Electric school bus success hinges on long-term planning, which means smart financing, battery management, and service-based models that keep fleets reliable for years.
Read More →
Alt-Fuel Moves: New V2G Tech and Electric Bus Rollouts
Check out some of the latest moves where alternative fuels and school buses intersect, including new product announcements and bus deployments across the U.S.
Read More →
Nuvve Strikes Deal to Electrify N.M. District School Buses
Nuvve’s latest partnership in New Mexico aims to help districts transition to electric school buses while strengthening local grid reliability.
Read More →
Electric School Buses: Progress, Promise, and the Practical Road Ahead
The push for electric school buses grows, but real-world hurdles mean districts are adopting EVs slowly and mixing them with diesel and propane.
Read More →
California Agency to Fund $78M in New Clean School Buses
South Coast AQMD plans to replace 286 older buses with newer models, plus accompanying infrastructure, across 35 districts in the South Coast Air Basin.
Read More →
Where EPA School Bus Funding Stands: CSBP, DERA, and Heavy-Duty Grants Update
One program ends, another looks to be reimagined, and the Clean School Bus Program is in a holding pattern — here’s where each EPA program stands and what to expect moving forward.
Read More →
West Aurora District 129 Launches 27 Electric School Buses Backed by Nearly $1M in ComEd EV Rebates
The Illinois district’s new electric bus fleet, supported by EPA grants, ComEd incentives, and Highland Electric Fleets, advances its commitment to sustainable school operations.
Read More →
